Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Volume 217, Issue -, Pages 70-78Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2017.12.010
Keywords
Elovl4-like; Fish oil; LC-PUFA; Soybean oil; Scylla paramamosain
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Funding
- National Natural Science Foundation of China [31172424]
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In this report, the full-length cDNA of fatty acyl Elov14-like elongase was cloned from the hepatopancreas of Scylla paramamosain by rapid-amplification of cDNA ends (RACE). To the best of our knowledge, this is the first report of Elov14-like elongase in crustaceans. The full-length cDNA of Elov14-like was 1119 bp, which included a 5'-terminal untranslated region (UTR) of 58 bp, a 3'-terminal UTR of 44 bp and an open reading frame (ORF) of 1017 bp encoding a polypeptide of 338 amino acids. Tissue distribution analysis revealed that Elov14-like transcripts are widely distributed in various organs, with high mRNA levels in the hepatopancreas and cranial ganglia. Further, Elov14-like transcriptional levels in hepatopancreas were up-regulated in proportion to the replacement of dietary fish oil (FO) with soybean oil (SO). The result showed that Elovl4-like transcripts increased about 0.83 and 1.12-fold respectively when SO constituted 80% and 100% of total oil (P < 0.05). These results may contribute to better understanding of the long-chain polyunsaturated fatty acids (LC-PUFA) bio-synthetic pathway and regulation mechanism in this species.
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