Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 167, Issue -, Pages 448-456Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2018.04.009
Keywords
Injectable hydrogel; Self-crosslinkable hydrogel; Tunable mechanical property; Cell encapsulation; Tissue engineering
Funding
- Science and Technology Support Program of Sichuan Province [2016SZ0008]
- Guangxi Key Research and Development Plan [GuikeAB16450003]
- 111 Project [B16033]
- National Natural Science Foundation of China [51403134]
- Guangxi Scientific Research and Technological Development Foundation [14125008-2-14]
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Injectable and self-crosslinkable hydrogels have drawn much attention for their potential application as cell delivery carriers to deliver cells to the injury site of arbitrary shape. In this study, injectable and self-crosslinkable hydrogels were designed and fabricated based on collagen type I (Col I) and activated chondroitin sulfate (CS-5NHS) by physical and chemical crosslinking without the addition of any catalysts. The physical properties of hydrogels, including mechanical properties, swelling and degradation properties, were investigated. The results demonstrated that the physical properties of hydrogels, especially the stiffness of hydrogels, were readily tuned by varying the degree of substitution (DS) of CS-sNHS without changing the concentration of collagen-based precursor. Chondrocytes were encapsulated into hydro gels to investigate the effects of hydrogels on the survival, proliferation and extracellular matrix (ECM) secretion of cells by FDA/PI staining, CCK-8 test and histological staining. The results suggested that all of these hydrogels supported the survival and ECM secretion of chondrocytes, while there was more ECM secretion around chondrocytes encapsulated in hydrogel Col I/CS-sNHS56% in which the DS of CS-sNHS was 56%. When the neutral precursor solution for hydrogel of Col I or Col ICS-sNHS56% was subcutaneously injected into SD rats, hydrogels both displayed acceptable biocompatibility in vivo. These results imply that these injectable and self-crosslinkable hydrogels are suitable candidates for applications in the fields of cell delivery and tissue engineering. (C) 2018 Elsevier B.V. All rights reserved.
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