4.7 Article

Chitosan nanoencapsulation of flavonoids enhances their quorum sensing and biofilm formation inhibitory activities against an E.coli Top 10 biosensor

Journal

COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 164, Issue -, Pages 125-133

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2018.01.019

Keywords

Antiquorum sensing; Quorum sensing; E. coli Top 10 biosensor; Autoinducer; Baicalein; Quercetin

Funding

  1. DAAD-NACOSTI [A/13/93803]

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Phytochemicals have been found to be promising alternatives to conventional antibiotic therapies for the control of bacterial infections, as they may entail less selective pressure and hence reduce the development of resistance. This study involved examining the inhibition of biofilm formation and of quorum sensing (QS), and the cytotoxicity on mammalian cells of two flavonoids, quercetin and baicalein, in free form and associated into chitosan-based nanocapsules. This was done by use of a transformed E. coli Top 10 biosensor strain, while the cytotoxicity was evaluated on MDCK-C7 cells. In free form, application both flavonoids exhibited slight inhibitory activity on the QS response and biofilm formation, a scenario that was improved positively upon encapsulation with chitosan (Mw similar to 115,000 g/mol and DA similar to 42%). The association efficiency of 99% (quercetin) and 87% (baicalein) was determined, and each formulation had an average diameter of 190 +/- 4 and 187 +/- 2 nm, and zeta (zeta) potential of +48.1 +/- 2.03 and +48.4 +/- 3.46 mV, respectively. Both types of systems were stable against aggregation in M9 and MEM media. The in vitro release kinetics data of both flavonoids seemed to be similar with only similar to 20% released over the first 5 h, or similar to 10% over the first 4 h, respectively, with subsequent sudden release increase up to similar to 40% in both cases. The free phytochemicals seemed to be cytotoxic to MDCK-C7 cells at higher doses, however, upon nanoen-capsulation, a cytoprotective effect was evidenced. We have gained proof-of-principle of the advantages of encapsulation of two bioactive flavonoids. (C) 2018 Published by Elsevier B.V.

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