Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 162, Issue -, Pages 415-419Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2017.12.011
Keywords
Lead ion; Silver staining; PS2.M aptamer; Naked eyes; Visual detection
Funding
- National Natural Science Foundation of China [81471697, 81771878]
- Yellow Crane Talent (Science & Technology) Program of Wuhan City [2016060101010044]
- Applied Basic Research Program of Wuhan City [2016060101010044]
- Fundamental Research Funds for the Central Universities (Hust) [2016YXMS253, 2017KFXKJC002]
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In this paper, visual detection of trace lead ion was established by aptamer and silver staining. The basic strategy was that aminated PS2.M aptamer was immobilized onto slide and formed stable G-quadruplex structure. PbS was generated by adding S2-, and it catalyzed subsequent silver staining reaction, through the silver staining amplification effect, the slide presented visible ash black. The gray value of slide after silver staining was analyzed and the semi-quantitative detection of Pb2+ in solution was realized. The results showed that optical darkness ratio (ODR) and logarithmic value of Pb2+ concentration had a good linear relationship (R-2 =0.951) over the range of 0.5-10 mu M. In addition, there was no obvious interference of other common metal ions for the detection, indicating that this method presented outstanding selectivity. And it was also used for qualitative and semi-quantitative determination of Pb2+ in soil sample successfully. (C) 2017 Elsevier B.V. All rights reserved.
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