Journal
FOOD CONTROL
Volume 56, Issue -, Pages 47-52Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2015.03.008
Keywords
Salmonella enterica set. Enteritidis; Rti-LAMP; Lettuce; Sodium dodecyl sulfate; SDS; Bentonite coated activated carbon
Categories
Ask authors/readers for more resources
A real-time loop amplified (Rti-LAMP) DNA assay system was developed for the rapid detection of low numbers of Salmonella enterica ser. Enteritidis (s. enterica) on the leaves of romaine lettuce without enrichment. The assay involved seeding 50 g portions of leaves with various numbers of S. enterica CFU. The lowest level of DNA consistently detected by the Rti-LAMP assay was that from 25 CFU per 50 g (0.5 CFU/g) of lettuce equivalent to the DNA from 6 CFU per Rti-LAMP reaction. A standard curve was generated by plotting the Tp values (min) against the log of 6, 25, 60 and 250 CFU of S. enterica seeded onto 50 g of lettuce. The entire assay could be completed in 3.5 h.7 Published by Elsevier Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available