Journal
FOOD CONTROL
Volume 53, Issue -, Pages 156-162Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2015.01.028
Keywords
Antimicrobials; Listeria innocua; Listeria monocytogenes; Combined effect; Carvacrol; Citral; Sublethal injury
Categories
Funding
- National Council of Technological and Scientific Development CNPq
- Embrapa Labex Europa
- Project AGL [2010-22206-Co2-01]
- FEDER programme
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The aim of this study was to evaluate the growth kinetics of Listeria innocua Serovar 6a (CECT 910) and Listeria monocytogenes Serovar 4b (CECT 4032) exposed to combinations of carvacrol and citral (0.0 mu L/mL (control), 0.050 mu L/mL of carvacrol and 0.075 mu L/mL of citral, 0.050 mu L/mL of carvacrol and 0.125 mu L/mL of citral, 0.085 mu L/mL of carvacrol and 0.075 mu L/mL of citral, and 0.085 mu L/mL of carvacrol and 0.125 mu L/mL of citral), with two initial inoculum concentrations, and also the occurrence of sublethal damage in these cell populations. The terpene combinations exhibited antibacterial activity against L innocua and L monocytogenes and the effects were dependent on the concentration of terpenes present in the culture medium (p <= 0.05). When terpene-treated L innocua and L. monocytogenes were incubated in TSB, significant differences in lag phase and growth rate were observed between low and high inoculum concentrations (p <= 0.05), indicating that the inoculum level should be taken into account in modeling studies. When bacterial cells were exposed to terpenes the proportion of sublethally injured cells increased with the increase in the terpene dose (p <= 0.05). In conclusion, all of these results show that carvacrol and citral can be used in combination at 25% of the MIC in order to control Listeria growth. (C) 2015 Elsevier Ltd. All rights reserved.
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