Journal
APPLIED OPTICS
Volume 54, Issue 32, Pages 9518-9527Publisher
OPTICAL SOC AMER
DOI: 10.1364/AO.54.009518
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- Royal Society of New Zealand Marsden Fund
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We develop a video tracking method that utilizes an interpolation-based normalized cross-correlation approach to track the position of microscopic spherical particles in three dimensions. Subnanometer resolution is demonstrated. The method does not assume that the particle's image is radially symmetric, making it useful for determining the position when particles are close and their images overlap. This is demonstrated in a study of the electrostatic and hydrodynamic interactions between a pair of beads in dual laser tweezers traps. (C) 2015 Optical Society of America
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