Journal
FOOD CHEMISTRY
Volume 183, Issue -, Pages 30-35Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2015.03.024
Keywords
Flavonol glycosides; Green tea; Oolong tea; Black tea; UHPLC; HPLC
Funding
- CAAS [CAAS-ASTIP-2014-TRICAAS]
- China Scholarship Council
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An UHPLC method for the determination of flavonol glycosides (FOG) from green and oolong tea vs. black tea has been developed for the first time. Sample clean-up method by means of polyamide column chromatography was optimized with multiple-step elution. Using UHPLC and HPLC with gradient elution and photodiode array detection, eighteen FOG compounds were determined with the aid of electrospray tandem mass spectrometry. These FOG compounds were qualified on both UHPLC and HPLC, and this UHPLC method successfully separated rutin (quercetin-3-O-rutinoside) and K-grg (kaempferol-3-O-glucorhamnoglucoside) while conventional HPLC method did not. The total amounts of FOG compounds in the tea samples were 2.32-5.67 g/kg dry weight (calculated as aglycones), and there is no significant difference for the total FOG content among green tea, oolong tea and black tea. However, kaempferol glycosides are more abundant in green teas, while oolong tea has more quercetin and myricetin glycosides. In black tea quercetin glycosides were most abundant. (C) 2015 Elsevier Ltd. All rights reserved.
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