Journal
FOOD CHEMISTRY
Volume 184, Issue -, Pages 80-89Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2015.03.047
Keywords
Optimization; Response surface methodology; Central composite rotatable design; Ultrasound-assisted system; Total phenolic content; Phosphomolybdenum assay; 2,2-Diphenyl-1-picrylhydrazyl radical scavenging activity
Funding
- Ministry of Higher Scientific Research and Information and Communication Technologies of Tunisia
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The optimization of antioxidant extraction conditions from a ripe edible fruits of Zizyphis lotus (L) with an ultrasound-assisted system was achieved by response surface methodology. The central composite rotatable design was employed for optimization of extraction parameters in terms of total phenolic content and antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and phosphomolybdenum assay. The optimum operating conditions for extraction were as follows: ethanol concentration, 50%; extraction time, 25 min; extraction temperature, 63 degrees C and ratio of solvent to solid, 67 mL/g. Under these conditions, the obtained extract exhibited a high content of phenolic compounds (40.782 mg gallic acid equivalents/g dry matter) with significant antioxidant properties (the total antioxidant activity was 75.981 mg gallic acid equivalents/g dry matter and the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity was 0.289 mg/mL). (C) 2015 Elsevier Ltd. All rights reserved.
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