Effects of alkaline pretreatments and acid extraction conditions on the acid-soluble collagen from grass carp (Ctenopharyngodon idella) skin

This study investigated the effects of alkaline pretreatments and acid extraction conditions on the production of acid-soluble collagen (ASC) from grass carp skin. For alkaline pretreatment, 0.05 and 0.1 M NaOH removed non-collagenous proteins without significant loss of ASC at 4, 10, 15 and 20 degrees C; while 0.2 and 0.5 M NaOH caused significant loss of ASC, and 0.5 M NaOH caused structural modification of ASC at 15 and 20 degrees C. For acid extraction at 4, 10, 15 and 20 degrees C, ASC was partly extracted by 0.1 and 0.2 M acetic acid, while 0.5 and 1.0 M acetic acid resulted in almost complete extraction. The processing conditions involving 0.05-0.1 M NaOH for pretreatment, 0.5 M acetic acid for extraction and 4-20 degrees C for both pretreatment and extraction, produced ASC with the structural integrity being well maintained and hence were recommended to prepare ASC from grass carp skin in practical application. (C) 2014 Elsevier Ltd. All rights reserved.