Journal
FOOD CHEMISTRY
Volume 168, Issue -, Pages 566-571Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2014.07.097
Keywords
Biopolymer particles; EGCG; Fluorescence; Binding constant; Stability
Funding
- National Natural Science Foundation of China [21173081]
- Fundamental Research Funds for the Central Universities [WK1213003]
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Novel thermally-induced BSA/iota-carrageenan particles are used as a protective carrier for (-)-epigallocatechin-3-gallate (EGCG). The addition of EGCG to BSA/iota-carrageenan particles can highly quench the intrinsic fluorescence of BSA, which is explained in terms of the binding of EGCG to the hydrophobic pockets of BSA mainly through the hydrophobic force. According to the double logarithm equation, the binding constant is determined as 1.1 x 10(8) M-1 for the binding of EGCG with BSA/iota-carrageenan particles. The high binding affinity is ascribed to both the molecular structure of EGCG and the partial unfolding state of BSA in BSA/iota-carrageenan particles. The circular dichroism spectra and calculated a-helix of BSA suggest that the bound EGCG leads to a more random secondary structure of BSA. Furthermore, BSA/iota-carrageenan particles are found to be superior to native BSA and pure BSA particles for improving the stability and radical scavenging activity of EGCG. (C) 2014 Elsevier Ltd. All rights reserved.
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