Journal
FOOD CHEMISTRY
Volume 184, Issue -, Pages 7-11Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2015.03.065
Keywords
Mycotoxin; Red yeast rice; Food analysis; Biosensor
Funding
- Pamukkale University
- Sinop University
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A novel and sensitive molecular imprinted surface plasmon resonance (SPR) biosensor was developed for selective determination of citrinin (CIT) in red yeast rice. Firstly, the gold surface of SPR chip was modified with allyl mercaptane. Then, CIT-imprinted poly(2-hydroxyethyl methacrylate-methacryloylamidoglutamic acid) (p(HEMA-MAGA)) film was generated on the gold surface modified with allyl mercaptane. The unmodified and imprinted surfaces were characterized by Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM) and contact angle measurements. The linearity range and the detection limit were obtained as 0.005-1.0 ng/mL and 0.0017 ng/mL, respectively. The SPR biosensor was applied to determination of CIT in red yeast rice sample. (C) 2015 Elsevier Ltd. All rights reserved.
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