Journal
FOOD CHEMISTRY
Volume 169, Issue -, Pages 65-72Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2014.07.131
Keywords
2D-PAGE; Beef cattle; Calcium; Mass spectrometry; Zebu genotype
Funding
- Sao Paulo Research Foundation (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, FAPESP) [2009/16118-5, 2010/51332-5, 2011/14850-0]
- Government Funding Agency (Coordenadoria de Aperfeicoamento de Pessoal de Nivel Superior - CAPES)
- Institute of Biosciences Foundation (FUNDIBIO)
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [09/16118-5, 11/14850-0] Funding Source: FAPESP
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The work describes a metalloproteomics study of bovine muscle tissue with different grades of meat tenderness from animals of the Nellore breed (Bos indicus) based on protein separation by two-dimensional gel electrophoresis, the identification of calcium ions in protein spots by X-ray fluorescence (SR-XRF) and the characterisation of proteins by electrospray ionisation mass spectrometry. Forty (40) specimens were selected and divided into two experimental groups: animals with tough meat (TO) and animals with tender meat (TE). A third group (P) of Piedmontese breed animals (Bos taurus) was included to serve as a comparative model for the level of meat tenderness. The procedures were efficient and preserved the metal protein structure, enabling calcium detection in protein spots by SR-XRF at a given molecular weight range of 14-97 kDa. Two proteins (pyruvate kinase and albumin) were inferred to be related to the phenotypical differences in animals from the different groups. (C) 2014 Elsevier Ltd. All rights reserved.
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