Journal
FOOD CHEMISTRY
Volume 173, Issue -, Pages 231-237Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2014.09.170
Keywords
Myristica fragrans; Neolignans; Nitric oxide; Inhibition; iNOS mRNA
Funding
- National Key Technologies R&D Program of China [2011BAI07B08]
- National Natural Science Foundation of China [30973863, 81161120429]
- NATIONAL CANCER INSTITUTE [ZIABC005708, ZIABC005562] Funding Source: NIH RePORTER
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Five new 8-0-4' type neolignans, named myrifralignan A-E (1-5), together with five known analogues (6-10), were isolated from the seeds of Myristica fragrans Houtt. Their chemical structures were determined using several spectroscopic methods. Compounds 3-10 exhibited potent inhibitory activity against the production of nitric oxide (NO) in the RAW264.7 cell line stimulated by lipopolysaccaride. Myrislignan (7) and machilin D (10) were the most potent inhibitors of NO production amongst these compounds. The IC50 values of myrislignan and machilin D were 21.2 and 18.5 mu M. And, their inhibitory activity was more than L-N-6-(1-iminoethyl)-lysine, a selective inhibitor of inducible nitric oxide synthase (IC50 = 27.1 mu M). Furthermore, real-time PCR analysis revealed that these neolignans could significantly suppress the expression of inducible nitric oxide synthase mRNA. These results demonstrated that the 8-0-4' type neolignans are promising candidates as anti-inflammatory agents. (C) 2014 Elsevier Ltd. All rights reserved.
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