4.1 Article

Elaboration and characterization of pectin-gellan films added with concentrated supernatant of Streptococcus infantarius fermentations, and EDTA: effects on the growth of Escherichia coli, Staphylococcus aureus and Listeria monocytogenes in a Mexican cheese medium, and physical-mechanical properties

Journal

FOOD SCIENCE AND TECHNOLOGY
Volume 39, Issue 2, Pages 436-443

Publisher

SOC BRASILEIRA CIENCIA TECNOLOGIA ALIMENTOS
DOI: 10.1590/fst.32717

Keywords

lactic acid bacterium; bacteriocins; biopreservation; mechanical properties; gas permeability

Funding

  1. CONACyT
  2. CONACyT-Mexico [230138, 254437, 269805]
  3. PRODEP-SEP-Mexico

Ask authors/readers for more resources

Films were prepared with 0.2% (w/v) gellan gum, 1% (w/v) citric pectin, 0.5% (w/v) glycerol, 5 mM CaCl2, 0.05 M Ethylenediaminetetraacetic acid (EDTA), and different concentrations of an antimicrobial activity-concentrated supernatant (AMC) obtained from fermentations of Streptococcus infantarius, which produces bacteriocin-like inhibitory substances (i.e., treatments F1, F2 and F3 with 75, 90 and 120 arbitrary units (AU)/mL, respectively). The treatments were based on a minimum-inhibitory-concentration (i.e., AMC, 90 AU/mL, plus EDTA, 0.05 M) for Escherichia coli, Staphylococcus aureus and Listeria monocytogenes growing in brain-heart-infusion medium at 35 degrees C. The films hindered the bacterium growth in selective media: E. coli-MacConkey, S. aureus-Baird Parker and L. monocytogenes-Oxford, during 30 days at 25 degrees C. Moreover, the F2 films entirely inhibited the growth of the tested bacteria in a Mexican cheese medium, in 7-day cultures at 35 degrees C; contrariwise, controls with no film exhibited bacterial growths in the range 10(7) -10(9) CFU/g. The physical-mechanical properties of the films were changed by the addition of EDTA-AMC, being (F2 film)/(control-film with no AMC nor EDTA): Young's modulus (MPa), 1,394/707; Elongation at break (%), 1.9/9.3; Stress at break (MPa), 5.7/12.6; Water Vapour Permeability (10(-11) g m Pa-1 s(-1) m(-2)) 3/20, and Oxygen Permeability (10(-12) g m Pa-1 s(-1) m(-2)), 1.9/1.2.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.1
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available