Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 84, Issue -, Pages 79-88Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2015.08.007
Keywords
Bisphenols; Peripheral blood mononuclear cells; Apoptosis; Caspase activation; Cytosolic calcium ions level; PARP-1 cleavage
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Funding
- Polish National Science Centre [2012/07/B/NZ7/01174]
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There are only a few studies that have assessed the effect of bisphenol A (BPA) on human blood cells and no study has been conducted to analyze the impact of BPA analogs on human leucocytes. In this study, we have investigated the effect of BPA and its analogs like bisphenol F (BPF), bisphenol S (BPS) and bisphenol AF (BPAF) on apoptosis induction in human peripheral blood mononuclear cells (PBMCs). In order to clarify the mechanism of bisphenols-induced programmed cell death, changes in various signaling molecules of this process have been assessed. We observed an increase in cytosolic calcium ions (Ca2+) level and reduction of transmembrane mitochondrial potential (Delta Psi m) in PBMCs incubated with all compounds examined, and particularly BPA and BPAF. All compounds studied changed PBMCs membrane permeability, activated caspase-8, -9, -3 and induced PARP-1 cleavage and chromatin condensation, which confirmed that they were capable of inducing apoptosis both via intrinsic and extrinsic pathway. Moreover, we have found that modus operandi of bisphenols studied was different. We noticed that BPAF and BPS caused mainly necrotic and apoptotic changes, respectively, whereas BPA induced comparable apoptotic and necrotic effects in the incubated cells. (C) 2015 Elsevier Ltd. All rights reserved.
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