Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 84, Issue -, Pages 188-196Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2015.08.030
Keywords
Mycotoxins; Flow cytomtery; Mitochondrial membrane potential; Cell cycle; Enniatins; Beauvericin
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Funding
- Ministry of Science and Innovation [AGL2010-17024]
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Hepatotoxicity of three Fusarium mycotoxins, beauvericin (BEA) and two enniatins (ENNs) ENN A1 and ENN B1, in hepatocarcinoma cells (HepG2) were evaluated and compared. Concentrations used were 1.5 and 3 mu M at 24, 48 and 72 h for each mycotoxin. Flow cytometry was used to examine enniatins effects on cell proliferation, to characterize the cell cycle phase where the cells blocked and to study the mitochondria role in ENNs-induced apoptosis. ENN B1 treated cells showed a time dependent G1 blockade at both concentrations used. ENN Al and BEA decreased the apoptotic-necrotic percentage of cells comparing to control and disrupted the MMP as observed by TMRM and ToPro-3 fluorochromes signal. It is proposed a decreasing mycotoxin order by number of effects as follows: BEA > ENN B1 > ENN Al, with 47, 20 and 16%, respectively out of all situations compared. (C) 2015 Elsevier Ltd. All rights reserved.
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