Impacts of different morphologies of anammox bacteria on nitrogen removal performance of a hybrid bioreactor: Suspended sludge, biofilm and gel beads

The difficulties in the anaerobic ammonium oxidation (anammox) process mainly consist of low microbial growth rates and long start-up times of bioreactors. The morphologies of anammox bacteria might affect nitrogen removal performance and microbial community. In this study, three morphologies of anammox bacteria, namely, suspended sludge, biofilm and suspended sludge embedded in gel beads, were compared in a hybrid bioreactor under anoxic conditions (DO concentration < 0.1 mg L-1). The results show that the average total inorganic nitrogen removal efficiency of a hybrid bioreactor reached 67 +/- 15% with a maximum value of 80% for continuous synthetic wastewater feeding, and that the specific total inorganic nitrogen removal rate reached 15.75 mg.(gVSS. h)(-1) regardless of the organic matter stress. Batch tests indicate that mainly suspended sludge (67%) and biofilm (26%) contributed to the anammox process, with the specific total inorganic nitrogen removal rate reaching 10.55 and 4.05 mg.(gVSS.h)(-1), respectively. However, the embedding of sludge in gel resulted in nitrification instead of anammox with a nitrification rate of 0.20 +/- 0.01 mg.(L.h)(-1) due to the expansion of gel beads floating on the water surface. Therefore, a pore-forming technique was developed to produce more channels for gas dispersion inside the gel beads. In terms of microbial community, Candidatus Kuenenia involved in the anammox group was the most abundant genus in biofilm (43.4%) and suspended sludge (15.7%), while Nitrospira occupied the largest proportion in gel beads (25.6%). This study offers useful information for the selection of anammox bacteria morphology. (C) 2018 Elsevier Ltd. All rights reserved.