Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 24, Issue 46, Pages 11885-11889Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201802799
Keywords
Coordination Chemistry; Glycocalyx; Lanthanide Luminescence; Microscopy; Spectroscopy
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Funding
- UNIK research initiative of the Danish Ministry of Higher Education Science [09-065274]
- Danish Council of Independent Research [DFF-1323-00352, DFF-6110-00554]
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Lanthanide(III) ions bind to the glycocalyx of Chinese Hamster Ovary (CHO) cells and give rise to a unique luminescent fingerprint. Following direct excitation of terbium(III) and europium(III) ions in the visible part of the spectrum, we are able to collect emission spectra pixel-by-pixel in images of CHO cells. Following data analysis that removes the background signal, the fine structure of the europium(III) luminescence indicate that the lanthanide(III) ions are bound to a single structure of the CHO cell glycocalyx. This was deduced from the fact that the structure-sensitive emission spectrum of europium is unchanged throughout the investigated samples.
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