Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 24, Issue 23, Pages 6025-6029Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201800085
Keywords
aggregation; biosensors; capillary; microfluidic device; pathogens
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Funding
- BIO5 Institute, The University of Arizona
- U.S. National Science Foundation (NSF) Water, Environment and Technology (WET) Center [IIP-1361815]
- Tucson Water
- NSF [DGE-1143953]
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Toward ultra-simple and field-ready biosensors, we demonstrate a novel assay transducer mechanism based on interfacial property changes and capillary flow dynamics in antibody-conjugated submicron particle suspensions. Differential capillary flow is tunable, allowing pathogen quantification as a function of flow rate through a paper-based microfluidic device. Flow models based on interfacial and rheological properties indicate a significant relationship between the flow rate and the interfacial effects caused by target-particle aggregation. This mechanism is demonstrated for assays of Escherichia coli K12 in water samples and Zika virus (ZIKV) in blood serum. These assays achieved very low limits of detection compared with other demonstrated methods (1 log CFU/mL E. coli and 20 pg/mL ZIKV whole virus) with an operating time of 30 s, showing promise for environmental and health monitoring.
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