Journal
FOOD AND AGRICULTURAL IMMUNOLOGY
Volume 27, Issue 4, Pages 449-459Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2015.1104657
Keywords
chicken tissue; ic-ELISA; immunochromatographic strip; ribavirin
Funding
- MOST [2012BAK08B01, 2012BAD29B04]
- Natural Science Foundation of Jiangsu Province
- MOF
- MOE [BK20140003, BE2013613, BE2013611]
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The use of ribavirin (RBV) as an antiviral drug for livestock has been prohibited in China, the USA, and many other countries. In this study, we developed a rapid and sensitive indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a gold nanoparticle immunochromatographic (ICA) strip test for detecting RBV in chicken muscles. Under the optimum assay conditions, where the assay employed phosphate-buffered saline at pH 7.4, no acetonitrile, and an ionic strength of 0.8%, the quantitative working range was 1.43-26.47ng/ml with an IC50 of 6.15ng/ml. The recovery rate for RBV in real samples ranged from 82.1% to 112.3%. The immunochromatographic test strip method had a visual cutoff value of 50g/kg. Given their high recovery rates and good sensitivity, the proposed ic-ELISA and ICA methods could be useful for the RBV analysis in chicken tissue samples.
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