Journal
FOOD ANALYTICAL METHODS
Volume 8, Issue 10, Pages 2496-2503Publisher
SPRINGER
DOI: 10.1007/s12161-015-0141-7
Keywords
Molecular imprinting; Hydrophilic imprinted membrane; Biomimetic antibody; Enzyme-linked immunosorbent assay; Multi-pesticide residues; Asparagus; Cucumber
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Funding
- National Natural Science Foundation of China [31471649]
- Agricultural Science and Technology Achievements Transformation Project of China [2013GB2C600276]
- National Natural Science Foundation of China
- Agricultural Science and Technology Achievements Transformation Project of China
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This study describes the development of a new direct competitive enzyme-linked immunosorbent assay method with a hydrophilic imprinted film as biomimetic antibody for determining multi-pesticide residues. The imprinted film, which can selectively recognize trichlorfon and acephate, was synthesized directly on the surface of a 96-well plate using 4-(dimethoxyphosphorothioylamino) butanoic acid as the template molecule. This film exhibited antibody-like recognition ability, fast adsorption-desorption dynamics and good stability. Under optimal conditions, the sensitivity (IC50) of the biomimetic enzyme-linked immunosorbent assay (BELISA) method was 12.0 mg/L for trichlorfon and 30.0 mg/L for acephate. The limit of detection (LOD, IC15) was 8.0 mu g/L for trichlorfon and 12.0 mu g/L for acephate. The developed method was applied to the determination of the trichlorfon and acephate in the spiked asparagus and cucumber samples with recoveries ranging from 72.1 to 92.0 % for trichlorfon and 70.0 to 85.0 % for acephate.
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