Journal
CHEMICAL COMMUNICATIONS
Volume 54, Issue 54, Pages 7491-7494Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8cc03268h
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We describe here a binding-facilitated reaction strategy, enabling quantitative conjugation of DNA to native proteins with a desirable 1:1 stoichiometry. The technique takes advantage of the iterative affinity interaction and covalent binding processes to achieve complete conjugation. The complete conjugation obviates the need for separation of the protein-DNA conjugates as required by other DNA-protein conjugation methods.
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