Journal
CHEMICAL COMMUNICATIONS
Volume 54, Issue 36, Pages 4569-4572Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8cc01521j
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Funding
- UNIK research initiative of the Danish Ministry of Science, Technology and Innovation [09-065274]
- bioSYNergy
- University of Copenhagen's Excellence Programme for Interdisciplinary Research
- Villum Foundation [VKR023115]
- Carlsberg Foundation [CF14-0388]
- Danish Council of Independent Research [DFF-7014-00027]
- Center for Synthetic Biology'' at Copenhagen University
- Villum Fonden [00007264] Funding Source: researchfish
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Measurements on biological samples are often hampered by auto-fluorescence from inherent compounds in tissue or cells, limiting the achievable contrast. Both the signal of interest and the auto-fluorescence are usually detected on the Stokes side of the excitation laser. In this communication, we present two new microscopy modalities, based on the emission of a red-emitting DNA-stabilized silver nanocluster (DNA-AgNC). Its bright fluorescence can be generated on the anti-Stokes side of the readout laser, allowing easy spectral separation of the signal of interest from the Stokes side auto-fluorescence.
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