4.7 Article

Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A

Journal

CHEMICAL COMMUNICATIONS
Volume 54, Issue 57, Pages 7892-7895

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c8cc02760a

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Funding

  1. Ministry of Education, Culture, Sports, Science, and Technology (Japan) [17H03087]
  2. JST CREST, Japan [JPMJCR15P3]
  3. JSPS KAKENHI [JP15H05806]
  4. Grants-in-Aid for Scientific Research [17H03087] Funding Source: KAKEN

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Haem substitution is an effective approach to tweak the function of haemoproteins. Herein, we report a facile haem substitution method for self-sufficient cytochrome P450BM3 (CYP102A1) from Bacillus megaterium utilising the transpeptidase Sortase A from Staphylococcus aureus. We successfully constructed Mn-substituted BM3 and investigated its catalytic activity.

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