Journal
CHEMICAL COMMUNICATIONS
Volume 54, Issue 23, Pages 2898-2901Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7cc08740c
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Funding
- Russian Science Foundation [17-14-01086, 14-50-00131]
- Russian Foundation for Basic Research [15-54-74003, 16-34-60175, 17-00-00214, 17-04-01690]
- DAAD Fellowship [57314022]
- Sergey Shpiz stipend
- National Foundation for Cancer Research (NFCR)
- DFG IRTG [1816]
- Russian Science Foundation [14-50-00131] Funding Source: Russian Science Foundation
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We designed a genetically encoded ratiometric fluorescent probe, SypHer3s, with enhanced brightness and optimized pKa, which responds to pH changes in different cellular compartments. SypHer3s was successfully utilized for imaging the pH dynamics in mitochondria of living neurons and in quantitative pH measurement in zebrafish embryos.
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