Journal
CHEMICAL COMMUNICATIONS
Volume 54, Issue 38, Pages 4786-4789Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8cc01661e
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Funding
- EPSRC
- University of Bath
- Prostate Cancer UK [PG14-009]
- Royal Society
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [2012R1A3A2048814]
- EPSRC [EP/R003939/1] Funding Source: UKRI
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Two 'turn on' TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 mu M limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 mu M limit of detection. No toxicity was found for TCFCl-GSH and a clear 'turn on' with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells.
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