Journal
CHEMBIOCHEM
Volume 19, Issue 10, Pages 1044-1048Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201800080
Keywords
microreactors; PCR technology; polymerase chain reaction; polymers; recombinant proteins
Funding
- National Natural Science Foundation of China [NSF 21372097]
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Protein-polymer microcapsules have attracted much attention, due to their special features and potential in biological use. How to make the most of this type of bio-abiotic hybrid material is an intriguing question. Nevertheless, several unsatisfactory technical issues significantly limited the application of these materials. For instance, introducing various biomolecules and crosslinking for the capsules remains challenging and problematic. In this report, recombinant mCherry protein was covalently linked with poly(N-isopropylacrylamide) (PNIPAAm) to form amphiphilic protein-polymer conjugates, which assembled into microcapsules. These microcapsules are thermoresistant and can be used in the polymerase chain reaction (PCR). In this setting, the reactant molecules can be readily and easily introduced into the microcapsules, and crosslinking and water-oil phase transition are not necessary. This protein-polymer microcapsule PCR system has potential in various biological applications.
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