Journal
CELLULAR SIGNALLING
Volume 42, Issue -, Pages 67-76Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cellsig.2017.10.005
Keywords
Fetuin A; SIRT1; AMPK; PGC1 alpha; Adipocyte; Energy homeostasis
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Funding
- UGC, New Delhi
- DST-SERB, New Delhi
- ICAR, New Delhi
- DST-SERB, New Delhi [SB/SO/AS/064/2013]
- Indian National Science Academy (INSA)
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Although several reports demonstrated that accumulation of excess lipid in adipose tissue produces defects in adipocyte which leads to the disruption of energy homeostasis causing severe metabolic problems, underlying mechanism of this event remains yet unclear. Here we demonstrate that FetuinA (FetA) plays a critical role in the impairment of two metabolic sensors, SIRT1 and AMPK, in inflamed adipocytes of high fat diet (HFD) mice. A linear increase in adipocyte hypertrophy from 10 to 16 week was in tandem with the increase in FetA and that coincided with SIRT1 cleavage and decrease in pAMPK which adversely affects PGC1 alpha activation. Knock down (KD) of FetA gene in HFD mice could significantly improve this situation indicating FetA's contribution in the damage of energy sensors in inflamed adipocyte. However, FetA effect was not direct, it was mediated through TNF-alpha which again is dependent on FetA as FetA augments TNF-alpha expression. FetA being an upstream regulator of TNF-alpha, its suppression prevented TNF-alpha mediated Caspase-1 activation and cleavage of SIRT1. FetA induced inactivation of PGC1 alpha due to SIRT1 cleavage decreased PPAR gamma, adiponectin, NRF1 and Tfam expression. All these together caused a significant fall in mitochondrial biogenesis and bioenergetics that disrupted energy homeostasis resulting loss of insulin sensitivity. Taken together, our findings revealed a new dimension of FetA, it not only induced inflammation in adipocyte but also acts as an upstream regulator of SIRT1 cleavage and AMPK activation. Intervention of FetA may be worthwhile to prevent metabolic imbalance that causes insulin resistance and type 2 diabetes.
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