Journal
CELLULAR IMMUNOLOGY
Volume 324, Issue -, Pages 74-77Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cellimm.2017.12.005
Keywords
CCR2; MCP-1; Transwell; AMNIS; THP-1; Cholesterol; M beta CD
Categories
Funding
- NIH [HL112629]
- National Institute on Minority Health and Health Disparities from the National Institutes of Health (NIH) [G12MD007591]
- San Jose State University
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Cholesterol content influences several important physiological functions due to its effect on membrane receptors. In this work, we tested the hypothesis that cellular cholesterol alters chemotactic response of monocytes to Monocyte Chemoattractant Protein-1(MCP-1) due to their effect on the receptor, CCR2. We used Methyl-beta-cyclodextrin (M beta CD) to alter the baseline cholesterol in human monocytic cell line THP-1, and evaluated their chemotactic response to MCP-1. Compared to untreated cells, cholesterol enrichment increased the number of monocytes transmigrated in response to MCP-1 while depletion had opposite effect. Using imaging flow cytomerry, we established that these differences were due to alterations in expression levels, but not the surface distribution, of CCR2.
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