Journal
CELL BIOLOGY INTERNATIONAL
Volume 42, Issue 5, Pages 570-579Publisher
WILEY
DOI: 10.1002/cbin.10933
Keywords
CD44; chondrogenesis; limb bud mesenchymal cells; mesenchymal stromal cells; osteogenesis; Sca1
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Funding
- Consejo Nacional de Ciencia y Tecnologia [168642]
- Direccion General Asuntos del Personal Academico (DGAPA)-Universidad Nacional Autonoma de Mexico [IN213314, IN211117]
- Fundacion Miguel Aleman, A. C., Mexico
- Consejo Nacional de Ciencia y Tecnologia (CONACyT)
- Programa de Doctorado en Ciencias Bioquimicas, Universidad Nacional Autonoma de Mexico
- Programa de Apoyo a Proyectos de Investigacion e Innovacion Tecnologica (PAPIIT) [IN211117]
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Skeletal progenitors are derived from resident limb bud mesenchymal cells of the vertebrate embryos. However, it remains poorly understood if they represent stem cells, progenitors, or multipotent mesenchymal stromal cells (MSC). Derived-MSC of different adult tissues under in vitro experimental conditions can differentiate into the same cellular lineages that are present in the limb. Here, comparing non-cultured versus cultured mesenchymal limb bud cells, we determined the expression of MSC-associated markers, the in vitro differentiation capacity and their gene expression profile. Results showed that in freshly isolated limb bud mesenchymal cells, the proportion of cells expressing Sca1, CD44, CD105, CD90, and CD73 is very low and a low expression of lineage-specific genes was observed. However, recently seeded limb bud mesenchymal cells acquired Sca1 and CD44 markers and the expression of the key differentiation genes Runx2 and Sox9, while Scx and Pparg genes decreased. Also, their chondrogenic differentiation capacity decreased through cellular passages while the osteogenic increased. Our findings suggest that the modification of the cell adhesion process through the in vitro method changed the limb mesenchymal cell immunophenotype leading to the expression and maintenance of common MSC-associated markers. These findings could have a significant impact on MSC study and isolation strategy because they could explain common variations observed in the MSC immunophenotype in different tissues.
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