Journal
CELL
Volume 172, Issue 4, Pages 881-+Publisher
CELL PRESS
DOI: 10.1016/j.cell.2018.01.020
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Funding
- Chinese Academy of Sciences (Key Research Program of Frontier Sciences) [QYZDY-SSW-SMC001]
- CAS Key Technology Talent Program
- Shanghai Municipal Government Bureau of Science and Technology Grant [16JC1420200]
- National Postdoctoral Program for Innovative Talents [BX201700266]
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Generation of genetically uniform non-human primates may help to establish animal models for primate biology and biomedical research. In this study, we have successfully cloned cynomolgus monkeys (Macaca fascicularis) by somatic cell nuclear transfer (SCNT). We found that injection of H3K9me3 demethylase Kdm4d mRNA and treatment with histone deacetylase inhibitor trichostatin A at one-cell stage following SCNT greatly improved blastocyst development and pregnancy rate of transplanted SCNT embryos in surrogate monkeys. For SCNT using fetal monkey fibroblasts, 6 pregnancies were confirmed in 21 surrogates and yielded 2 healthy babies. For SCNT using adult monkey cumulus cells, 22 pregnancies were confirmed in 42 surrogates and yielded 2 babies that were short-lived. In both cases, genetic analyses confirmed that the nuclear DNA and mitochondria DNA of the monkey offspring originated from the nucleus donor cell and the oocyte donor monkey, respectively. Thus, cloning macaque monkeys by SCNT is feasible using fetal fibroblasts.
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