Journal
CELL
Volume 172, Issue 4, Pages 771-+Publisher
CELL PRESS
DOI: 10.1016/j.cell.2017.12.027
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Funding
- European Research Council (ERC) [260822]
- Agence Nationale pour la Recherche [HiResBac ANR-15-CE11-0023-03, ANR-12-BSV8-0020-01]
- Fondation pour la Recherche Medicale
- Agence Nationale de la Recherche (ANR) [ANR-12-BSV8-0020] Funding Source: Agence Nationale de la Recherche (ANR)
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As in eukaryotes, bacterial genomes are not randomly folded. Bacterial genetic information is generally carried on a circular chromosome with a single origin of replication from which two replication forks proceed bidirectionally toward the opposite terminus region. Here, we investigate the higher-order architecture of the Escherichia coli genome, showing its partition into two structurally distinct entities by a complex and intertwined network of contacts: the replication terminus (ter) region and the rest of the chromosome. Outside of ter, the condensin MukBEF and the ubiquitous nucleoid-associated protein (NAP) HU promote DNA contacts in the megabase range. Within ter, the MatP protein prevents MukBEF activity, and contacts are restricted to similar to 280 kb, creating a domain with distinct structural properties. We also show how other NAPs contribute to nucleoid organization, such as H-NS, which restricts short-range interactions. Combined, these results reveal the contributions of major evolutionarily conserved proteins in a bacterial chromosome organization.
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