Depside derivatives with anti-hepatic fibrosis and anti-diabetic activities from Impatiens balsamina L. flowers

Eighteen compounds (1-18), seven new (3-9) and eleven previously reported (1, 2, and 10-18), were isolated from the flowers of Impatiens balsamina (Linn). The structures of the isolated compounds were elucidated using different spectroscopic methods, including NMR (1D and 2D), UV, IR, and HR-ESI-MS. Analysis of the bioassay results showed the compounds had notable anti-hepatic fibrosis activity against murine Hepatic Stellate Cells (t-HSC/CI-6) and anti-diabetics activity against a-glucosidase. Specifically, new compounds 7, 8, 9 showed significant inhibitory activity on t-HSC/CI-6 cells with IC50 values of 42.12, 109.2, and 34.04 mu g/mL respectively, while the IC50 values of positive control Silymarin and Fufang Biejia Ruangan Plan were 202.34 and 231.56 mu g/mL, respectively. In addition, compounds 2, 4, 7, 8, 10, 11, 17, and 18 exhibited excellent a-glucosidase inhibitory activity. Among these compounds, 7 exhibited the highest activity with an IC50 value of 0.72 mu g/mL, while the IC50 value of the positive control acarbose was 336 mu g/mL. This is the first study evaluating the anti-hepatic fibrosis and anti-diabetic activities of compounds isolated from the flowers of I. balsamina. (C) 2015 Elsevier B.V. All rights reserved.