Journal
CARBOHYDRATE POLYMERS
Volume 189, Issue -, Pages 178-183Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2018.01.096
Keywords
Laccase; TEMPO; Carboxylate groups; Chitin nanocrystal
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Funding
- National Natural Science Foundation of China [31100426]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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Laccase mediator oxidation was applied to chitin at pH 6.8 and 30 degrees C to prepare chitin nanocrystals with a catalytic amount of 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO). When 40 mM TEMPO and a total of 500 U laccase were added to 1 g chitin, the yield of water-insoluble oxidized chitin was more than 95%, and the carboxylate content was 0.43 mmol/g. Adsorption of laccase molecules on chitin particles occurred in a buffer at pH 6.8, which may have been caused by electrostatic interactions between positively charged C2-ammonium groups of chitin and anionically charged groups of laccase. Rod-like chitin nanocrystals (ChNCs) were obtained with average lengths and widths of 480 +/- 200 nm and 24 +/- 17 nm, respectively, by sonication of the oxidized chitin/water suspensions. The O-2/laccase/TEMPO oxidation caused no decrease in the degree of N-acetylation or the crystallinity of the original chitin based on FTIR and X-ray diffraction data.
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