Journal
CARBOHYDRATE POLYMERS
Volume 184, Issue -, Pages 427-434Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2017.12.069
Keywords
Alginate oligosaccharides; Amidation; Multivalency; Lectin; Alginate lyases
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Funding
- Brittany Regional Council
- OSEO as part of Poly-merproject [FUI-AAP14]
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Alginate oligosaccharides (AOS) with a weight average molecular weight of 5 kDa were efficiently amidated with amino acids and carbohydrates in aqueous media in the presence of 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM). Here, alanine, leucine, serine, as well as mannose and rhamnose, were amidated at high yields with a good control of the degree of substitution (DS). Amino acid-and carbohydrate-grafted AOS showed improved stability against degradation by alginate lyases having different specificities. This enzyme resistance was correlated with the DS: hydrolysis was reduced by 60-70% for low DS (0.1), whereas AOS with DS ranging from 0.4 to 0.6 remained unhydrolyzed. Competitive inhibition assays demonstrated multivalent binding of mannose-amidated AOS to concanavalin A lectin. A 178-fold affinity enhancement was observed for AOS(Man-0.38) (DS 0.38) over alpha-methyl-mannoside with an IC50 of 5.6 mu M, lending further evidence for the promising potential of AOS as multivalent scaffolds.
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