Journal
CARBOHYDRATE POLYMERS
Volume 182, Issue -, Pages 245-253Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2017.11.031
Keywords
Nanocomposite; Mesoporous material; Chitosan; Ionic liquid; Enzyme immobilization
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Funding
- National Natural Science Foundation of China [21676143]
- Qing Lan Project of Jiangsu Province
- Natural Science Foundation of Jiangsu Higher Education Institutions of China [14KJB530002]
- Self-Owned Research Project from Key Laboratory of Material-Oriented Chemical Engineering [ZK201603]
- Program for Innovative Research Team in University of Jiangsu Province
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A nanocomposite (SBA-CIL-CS) which was combined mesoporous silica SBA-15 material with chitosan via ionic liquid as the bridging agent was successfully fabricated. The morphology and structure of the nanocomposite were characterized in detail with transmission electron microscope, fourier transform infrared spectroscopy, thermogravimetric analysis and nitrogen adsorption-desorption techniques. SBA-CIL-CS was investigated as an efficient support for immobilization of porcine pancreas lipase (PPL) and possessed high immobilization efficiency. The properties of immobilized enzyme (SBA-CIL-CS-PPL) such as activity, stability and reusability have been significantly improved, and a preferable pH and temperature tolerance were obtained as well. Results demonstrated the inorganic-organic nanocomposite could be used as an ideal support for enzyme immobilization.
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