Journal
CARBOHYDRATE POLYMERS
Volume 192, Issue -, Pages 84-94Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2018.03.044
Keywords
Aloe vera; Chitosan; Lymphocyte; Fibroblast; Cell viability; Polyelectrolyte complex
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Funding
- Consejo Nacional de Ciencia y Tecnologia (CONACyT) [237292]
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Chitosan, sodium alginate and gel of Aloe vera (Aloe barbadensis Miller) were employed for the preparation of polyelectrolyte complexes at pH 4 and 6. FT-IR spectroscopy analysis showed evidence on complexes formation and incorporation of the Aloe vera gel. The zeta potential determination of the polyelectrolyte complexes revealed the presence of surface charges in the range of -20 to -24 mV, which results in stable systems. The dynamic moduli exhibited a high dependence on angular frequency, which is commonly found in solutions of macromolecules. The materials showed human fibroblast and lymphocyte viabilities up to 90% in agreement with null cytotoxicity. The polyelectrolyte complexes at pH 6 with Ca2+ were stable, showed high water absorption, satisfactory morphology, pore size and rigidity, characteristics that allowed significant human fibroblast migration in wound closure in vitro assays.
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