Journal
CANCER SCIENCE
Volume 109, Issue 4, Pages 912-918Publisher
WILEY
DOI: 10.1111/cas.13544
Keywords
bioluminescence imaging; fluorescence imaging; metastasis; tumor microenvironment; two-photon excitation microscopy
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Funding
- Japan Society for the Promotion of Science (JSPS KAKENHI) [JP15H04962, JP16K18578]
- MEXT/JSPS KAKENHI [JP15H05952, JP16H06280]
- Grants-in-Aid for Scientific Research [16K18578] Funding Source: KAKEN
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In vivo optical imaging using fluorescence and bioluminescence is superior to other methods in terms of spatiotemporal resolution and specificity, and represents a new technology for comprehensively studying living organisms in a less invasive way. Nowadays, it is an indispensable technology for studying many aspects of cancer biology, including dynamic invasion and metastasis. In observations of fluorescence or bioluminescence signals in a living body, various problems were caused by optical characteristics such as absorption and scattering and, therefore, observation of deep tissue was difficult. Recent developments in techniques for observation of the deep tissues of living animals overcame this difficulty by improving bioluminescent proteins, fluorescent proteins, and fluorescent dyes, as well as detection technologies such as two-photon excitation microscopy. In the present review, we introduce these technological developments and invivo application of bioluminescence and fluorescence imaging, and discuss future perspectives on the use of invivo optical imaging technology in cancer research.
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