Journal
CANCER LETTERS
Volume 415, Issue -, Pages 73-85Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2017.11.037
Keywords
Lung cancer; 20 (R)-Ginsenoside; Hypoxia; Epithelial-mesenchymal transition; Sternness
Categories
Funding
- National Natural Science Foundation of China [81572881, 81602508]
Ask authors/readers for more resources
Cisplatin is a first line chemotherapy in lung cancer, but decreased susceptibility may limit its application. In solid tumors, hypoxia alters the microenvironment and is associated with proliferation, metastasis, and drug sensitivity. The hypoxia-induced desensitization of cisplatin is not clearly elucidated. 20 (R)-Ginsenoside (Rg3), the traditional Chinese medicine, is extracted from ginseng and has antitumor activities. In this study, we evaluated if Rg3 is effective in improving cisplatin sensitivity by blocking hypoxia. We found that the inhibition of proliferation potential by cisplatin was reduced in cobalt chloride (CoCl2)-induced hypoxia in lung cancer cells. Hypoxia caused alterations in epithelial-mesenchymal transition (EMT), which were detected by cellular morphology and EMT protein markers, and in sternness analyzed by spheroid formation and marker molecules. Hypoxia also activated EMT, which was mediated by the nuclear factor kappa B (NF-kappa B) pathway, and sternness, and Rg3 inhibited the activation of the NF-kappa B pathway. Furthermore, Rg3 could increase the sensitivity to cisplatin by inhibiting EMT and sternness in hypoxic lung cancer cells, and this effect was confirmed in vivo. In conclusion, Rg3 may improve the sensitivity of cisplatin in lung cancer therapy. (C) 2017 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available