Journal
FISH PHYSIOLOGY AND BIOCHEMISTRY
Volume 41, Issue 6, Pages 1491-1500Publisher
SPRINGER
DOI: 10.1007/s10695-015-0101-6
Keywords
Carbonic anhydrase; Na+-K+-ATPase; Alkalinity stress; Oryzias latipes; Quantitative real-time PCR
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Funding
- Foundation of Key Laboratory of Yangtze River Water Environment, Ministry of Education (Tongji University), China [YRWEF201301]
- Special Scientific Research Funds for Central Non-profit Institutes (East China Sea Fisheries Research Institute) [2009M03]
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High carbonate alkalinity is one of the major stress factors for living organisms in saline-alkaline water areas. Acute and chronic effects of carbonate alkalinity on expression of two genes, carbonic anhydrase 2-like (CA2-like) and Na+-K+-ATPase alpha subunit (NKA-alpha) mRNA in medaka (Oryzias latipes) were evaluated to better understand the responses important for coping with a carbonate alkalinity stress. In the acute exposure experiment, the expression of CA2-like and NKA-alpha mRNA in the gill and kidney of medaka were examined from 0 h to 7 days exposed to 30.4 mM carbonate alkalinity water. Exposure to high carbonate alkalinity resulted in a transitory alkalosis, followed by a transient increase in gill and kidney CA2-like and NKA-alpha mRNA expression. In the chronic exposure experiment, the expression of these two genes was examined in the gill and kidney at 50 days post-exposure to six different carbonate alkalinity concentrations ranging from 1.5 to 30.4 mM. Gill and kidney CA2-like mRNA levels in 30.4 mM were approximately 10 and 30 times higher than that of the control (1.5 mM), respectively. Less differences were found in NKA-alpha expression in the 50-days exposure. The results indicate that when transferred to high carbonate alkalinity water, a transitory alkalosis may occur in medaka, followed by compensatory acid-base and ion regulatory responses. Thus, CA2-like and NKA-alpha are at least two of the important factors that contribute to the regulation of alkalinity stress.
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