Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 47, Issue 1, Pages 595-605Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2015.09.042
Keywords
Striped catfish; beta-glucan; Edwardsiella ictaluri; Immune gene expression
Funding
- Novus International
- Office of the Higher Education Commission (OHEC) Thailand
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Pangasianodon hypophthalmus (striped catfish) is an important aquaculture species and intensification of farming has increased disease problems, particularly Edwardsiella ictaluri. The effects of feeding beta-glucans on immune gene expression and resistance to E. ictaluri in P. hypophthalmus were explored. Fish were fed 0.1% fungal-derived beta-glucan or 0.1% commercial yeast-derived beta-glucan or a basal control diet without glucan. After 14 days of feeding, the mRNA expression of immune genes (transferrin, C-reactive protein, precerebellin-like protein, Complement C3 and factor B, 2a MHC class II and interleukin-1beta) in liver, kidney and spleen were determined. Following this fish from each of the three diet treatment groups were infected with E. ictaluri and further gene expression measured 24 h post-infection (h.p.i.), while the remaining fish were monitored over 2 weeks for mortalities. Cumulative percentage mortality at 14 days post-infection (d.p.i.) was less in beta-glucan fed fish compared to controls. There was no difference in gene expression between dietary groups after feeding for 14 days, but there was a clear difference between infected and uninfected fish at 24 h.p.i., and based on principal component analysis beta-glucans stimulated the overall expression of immune genes in the liver, kidney and spleen at 24 h.p.i. (c) 2015 Elsevier Ltd. All rights reserved.
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