Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 46, Issue 2, Pages 206-216Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2015.06.024
Keywords
TAB2; Antimicrobial peptide (AMP); Vago; Litopenaeus vannamei; WSSV; Vibrio parahaemolyticus
Funding
- National Natural Science Foundation of China [31402321, U1131002]
- China Postdoctoral Science Foundation [2014M552266]
- Chinese Agriculture Research system [CARS-47]
- National High Technology Research and Development Program of China (973 Program) [2012CB114401]
- Special Fund for Agro-scientific Research in the Public Interest [201103034]
- Foundation from Science and Technology Bureau of Guangdong Province [2011A020102002, 2009A020102002]
- Administration of Ocean and Fisheries of Guangdong Province [A201101B02]
- State Key Laboratory of Biocontrol [SKLBC09K06]
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In Drosophila, TAB2, an important intermediate in the IMD signaling pathway, plays critical roles in the innate immune response in response to bacterial and viral infection. However, the role of TAB-related proteins in the immune response of shrimp has not yet been established. Here, we reported the identification of a TAB2-like gene in Litopenaeus vannamei designated as LvTAB2. The full-length cDNA of LvTAB2 was 2160 bp with an open reading frame of 1827 bp, which encoded a putative protein of 608 amino acids including a ubiquitin binding domain (CUE) at the N-terminal and a Zinc Finger domain (ZnF) at the C-terminus. Real-time RT-PCR analysis showed that LvTAB2 was expressed in all tested tissues and the expression levels of LvTAB2 in gills and hemocytes were positively induced in response to LPS, Vibrio parahemolyticus and White Spot Syndrome Virus (WSSV) challenges. Dual luciferase reporter assays demonstrated that LvTAB2 was able to induce the expression of antimicrobial peptide (AMP) genes, including Drosophila Attacin A and shrimp Penaeidins. Interestingly, over-expression of LvTAB2 could up-regulate the promoter activities of L. vannamei Vago1, Vago3 and Vago4 genes in S2 cells. To our knowledge, it was the first report that TAB2 participated in innate immune signaling to regulate the expression of Vago genes in invertebrates. Moreover, RNAi-mediated knockdown of LvTAB2 enhanced sensitivity of L. vannamei to Vibrio parahaemolyticus infection and caused elevated virus loads after WSSV infection. We suggested that the LvTAB2 may play important roles in the shrimp innate immunity. (C) 2015 Elsevier Ltd. All rights reserved.
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