Journal
INTERNATIONAL JOURNAL OF SURGERY CASE REPORTS
Volume 10, Issue -, Pages 129-133Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ijscr.2015.03.046
Keywords
Mucosa-associated lymphoid tissue lymphoma; Oral cavity; Polymerase chain reaction; Immunoglobulin heavy chain gene rearrangement
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Funding
- Grants-in-Aid for Scientific Research [25861912] Funding Source: KAKEN
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INTRODUCTION: Intraoral mucosa-associated lymphoid tissue (MALT) lymphoma is a rare lymphoma that has a good prognosis if diagnosed correctly and treated in time. PRESENTATION OF CASE: A 64-year-old woman was referred to our department with asymptomatic swelling of the left hard palate. Computed tomography and magnetic resonance imaging revealed a mass in the left hard palate. We performed a pre-surgery biopsy; however, it was difficult to differentiate MALT lymphoma from other reactive lymphoproliferative disorders via gross or microscopic examination. Although the lesion was completely excised, histological findings did not allow a definitive diagnosis due to an absence of visible monoclonality. We then performed polymerase chain reaction (PCR) using DNA extracted from formalin-fixed, paraffin-embedded surgical samples. Capillary electrophoresis showed monoclonal peaks of immunoglobulin heavy chain gene rearrangement, thus facilitating a definitive diagnosis of MALT lymphoma. DISCUSSION: PCR technique is rapid, accurate, and enables a definitive diagnosis without relying on traditional histological or molecular diagnostic techniques, such as Southern blotting. CONCLUSION: We suggest that, if histological examination is ambiguous or fresh material is insufficient, PCR can be performed using paraffin-embedded materials to definitively diagnose low-grade lymphomas, such as MALT lymphoma. (C) 2015 The Authors. Published by Elsevier Ltd. on behalf of Surgical Associates Ltd.
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