Journal
BRAZILIAN JOURNAL OF MICROBIOLOGY
Volume 49, Issue 2, Pages 248-257Publisher
SPRINGER
DOI: 10.1016/j.bjm.2017.08.005
Keywords
Caves; 16S ribosomal RNA; Microbiota; Antimicrobial; Sediments
Categories
Funding
- Deanship of Scientific Research (DSR), King Abdulaziz University, Jeddah [141-003-D1434]
- DSR
Ask authors/readers for more resources
In this study for the first-time microbial communities in the caves located in the mountain range of Hindu Kush were evaluated. The samples were analyzed using culture-independent (16S rRNA gene amplicon sequencing) and culture-dependent methods. The amplicon sequencing results revealed a broad taxonomic diversity, including 21 phyla and 20 candidate phyla. Proteobacteria were dominant in both caves, followed by Bacteroidetes, Actinob acteria, Firmicutes, Verrucomicrobia, Planctomycetes, and the archaeal phylum Euryarchaeota. Representative operational taxonomic units from Koat Maqbari Ghaar and Smasse-Rawo Ghaar were grouped into 235 and 445 different genera, respectively. Comparative analysis of the cultured bacterial isolates revealed distinct bacterial taxonomic profiles in the studied caves dominated by Proteobacteria in Koat Maqbari Ghaar and Firmicutes in Smasse-Rawo Ghaar. Majority of those isolates were associated with the genera Pseudomonas and Bacillus. Thirty strains among the identified isolates from both caves showed antimicrobial activity. Overall, the present study gave insight into the great bacterial taxonomic diversity and antimicrobial potential of the isolates from the previously uncharacterized caves located in the world's highest mountains range in the Indian sub-continent. (C) 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available