Journal
BIOTECHNOLOGY JOURNAL
Volume 13, Issue 9, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201700601
Keywords
gene regulation; genome engineering; metabolic engineering; Saccharomyces cerevisiae; synthetic biology
Funding
- U.S. Department of Energy [DE-SC0018260, DE-SC0018420]
- Shen Postdoc Fellowship from University of Illinois at Urbana-Champaign
- Fundamental Research Funds for the Central Universities
- Startup Fund from Zhejiang University
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Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly used in the design and engineering of Saccharomyces cerevisiae, one of the most popular hosts for industrial biotechnology. This review summarizes the recent development of this disruptive technology for metabolic engineering applications, including CRISPR-mediated gene knock-out and knock-in as well as transcriptional activation and interference. More importantly, multi-functional CRISPR systems that combine both gain- and loss-of-function modulations for combinatorial metabolic engineering are highlighted.
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