Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 115, Issue 3, Pages 785-790Publisher
WILEY
DOI: 10.1002/bit.26505
Keywords
lignocellulosic sugars; metabolic redirection; synergetic carbon utilization; trehalose
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Funding
- National High Technology Research and Development Program of China [2015AA021001]
- Program of Introducing Talents of Discipline to Universities [B13005]
- Fundamental Research Funds for the Central Universities [YS1407, ZY1629, buctrc201613]
- National Natural Science Foundation of China [21306017, 21406010, 21636001]
- Program for Changjiang Scholars and Innovative Research Team in Universities in China [IRT13045]
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Trehalose, a multi-functional and value-added disaccharide, can be efficiently biosynthesized from glucose by using a synergetic carbon utilization mechanism (SynCar) which coupled phosphoenolpyruvate (PEP) generation from the second carbon source with PEP-dependent phosphotransferase system (PTS) to promote non-catabolic use of glucose. Considering glucose and xylose present in large amounts in lignocellulosic sugars, we explored new strategies for conversion of both sugars into trehalose. Herein, we first attempted trehalose production from xylose directly, based on which, synergetic utilization of glucose, and xylose prompted by SynCar was implemented in engineered Escherichia coli. As the results, the final titer of trehalose reached 5.55g/L in shake flask experiments. The conversion ratio or utilization efficiency of glucose or xylose to trehalose was around fourfold higher than that of the original strain (YW-3). This work not only demonstrated the possibility of directly converting xylose (C5 sugar) into trehalose (C12 disaccharide), but also suggested a promising strategy for trehalose production from lignocellulosic sugars for the first time.
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