Journal
BIOTECHNOLOGY ADVANCES
Volume 36, Issue 1, Pages 247-263Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2017.11.007
Keywords
Baeyer-Villiger monooxygenase; Protein engineering; Hot spots; Enantioselectivity; Substrate scope; Regioselectivity; Heteroatom oxidation; Cofactor usage; Uncoupling; Protein stability
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Funding
- Deutsche Bundesstiftung Umwelt [AZ 20013/231]
- German Federal State Mecklenburg-Vorpommern through a state graduate scholarship (Landesgraduiertenstipendium)
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Baeyer-Villiger monooxygenases (BVMOs) are versatile biocatalysts for the conversion of ketones to lactones or esters while also being able to efficiently oxidize sulfides to sulfoxides. However, there are limitations for the application of BVMOs in synthesis. In this review we provide an overview of the protein engineering studies aiming at optimizing different properties of BVMOs. We describe hot spots in the active sites of certain BVMOs that have been successfully targeted for changing the substrate scope, as well as the possibility to influence this property by allosteric effects. The identified hot spots in the active sites for controlling enantio- and regios-electivity are shown to be transferable to other BVMOs and we describe concepts to influence heteroatom oxidation, improve protein stability and change the cofactor dependency of BVMOs. Summarizing all these different studies enabled the identification of BVMO- or property-dependent as well as universal hot spots.
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