Journal
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
Volume 32, Issue 2, Pages 420-426Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/13102818.2017.1420425
Keywords
Cellulose; cellulase; nitrogen source; Lactococcus lactis subsp; lactis; simultaneous saccharification fermentation
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The aim of this work was to demonstrate the feasibility of nisin and lactic acid production from corn stover through simultaneous saccharification and fermentation using Lactococcus lactis subsp. lactis ATCC 11454. The optimum conditions for nisin and lactic acid production were cellulose concentration of 30g/L, a cellulase loading of 25 FPU/g cellulose and an initial pH of 6.0 in a 10g/L yeast extract and 10g/L peptone broth. The optimized bioprocess achieved nisin titer of 1635 IU/mL and lactic acid concentration of 15.2g/L. The results indicated that simultaneous saccharification and fermentation with corn stover is a promising bioprocess for the economic production of nisin and lactic acid simultaneously.
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