Journal
BIOSENSORS & BIOELECTRONICS
Volume 118, Issue -, Pages 160-166Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.07.029
Keywords
Electrochemiluminescence; Ratiometric, amplification; Metal-organic framework; Prostatic specific antigen
Categories
Funding
- National Natural Science Foundation of China [21375043]
- Sci-tech Innovation Foundation of Huazhong Agricultural University [2662017PY042]
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Signal-amplified ratiometric electrochemiluminescence (Sa-RECL) provides an attractive approach to maximize signal-to-noise ratio through enhancing signals and eliminate interferences. In this work, we prepared a novel metal-organic framework (MOF)/Au/G-quadruplex as both quenchers and enhancers to fabricate a target-triggered ratiometric ECL sensor for high sensitive and accurate detection of prostatic specific antigen (PSA). The ratiometric ECL sensor using the dual-potential-dependent ECL emitters (quantum dots (QDs) and luminol) and MOF/Au/G-quadruplex not only achieved signal self-calibration but also realized cooperative amplification. After the sequential hybridization among of complementary DNA-QDs, PSA aptamer and pDNA-Au-Hemin-MIL-DNAzyme and the further competition of PSA, the pDNA-Au-Hemin-MIL-DNAzyme probe would keep away from the electrode surface, causing a switchover of their ECL signals from off-on state to on-off state. The ratiometric ECL aptasensor exhibits high-sensitive and accurate analytical performance toward PSA with a linear detection range from 0.5 to 500 ng mL(-1) and a detection limit of 0.058 ng mL(-1) (S/N = 3). The novel ratiometric ECL biosensor has been successfully applied to determine different serum samples of PSA, indicating its potential application in the clinical diagnosis.
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